SnapShot: The Splicing Regulatory Machinery

SnapShot: The Splicing Regulatory Machinery

Sam68 Khdrbs1 KH A/U-rich Bcl-x; −/− increased osteoblast differentiation, reduced adipocyte differentiation Low High Expression in mouse

SnapShot: The ESCRT Machinery

SnapShot: Endogenous RNAi Machinery and Mechanisms

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Stochastic noise in splicing machinery

The number of known alternative human isoforms has been increasing steadily with the amount of available transcription data. To date, over 100 000 isoforms have been detected in EST libraries, and at least 75% of human genes have at least one alternative isoform. In this paper, we propose that most alternative splicing events are the result of noise in the splicing process. We show that the num...

Functional splicing network reveals extensive regulatory potential of the core spliceosomal machinery.

Pre-mRNA splicing relies on the poorly understood dynamic interplay between >150 protein components of the spliceosome. The steps at which splicing can be regulated remain largely unknown. We systematically analyzed the effect of knocking down the components of the splicing machinery on alternative splicing events relevant for cell proliferation and apoptosis and used this information to recons...